Rabbit Holes are the home and spawning place for Rabbits. They run back to their holes at dusk, or when they are frightened. When a Rabbit is captured or killed, another will eventually respawn from the hole. A Rabbit Hole can be dug up with a Shovel to force a Rabbit out; however, this will destroy the hole, preventing future Rabbits from spawning there. If a Rabbit is caught outside its hole after dark for any reason, it will fall asleep.
A Rabbit Hole will spawn a new rabbit every 2.5 days. Since a Morsel restores 12.5 hunger and the necessary calorie value per day is 75, this means that having access to 15 Rabbit Holes would be enough to sustain oneself through winter ([75/12]*2.5=15). It is also of note that a Rabbit that manages to flee into a Rabbit Hole after e.g a failed hunting attempt will not trigger the 2.5 days respawn timer. Instead, it will occupy the Rabbit Hole and leave it again (if the conditions are met) after a random amount of time between 1 and 3 real-time minutes.
Reign of Giants
“The Kingdom of the Bunnymen is closed for the season.”
In the Reign of Giants DLC, during Spring, Rabbit Holes will turn into Collapsed Rabbit Holes and won't spawn Rabbits anymore. A few days after Spring has ended, Collapsed Rabbit Holes will go back to normal and start spawning Rabbits again. A Collapsed Rabbit Hole can be dug up as well to force a Rabbit out, this will also destroy the hole forever.
Don't Starve Together
In Don't Starve Together, digging up and destroying a Rabbit Hole will cause the game to internally trigger a Regrowth Event, which allows for the Rabbit Hole to regenerate itself after 5 days. This effectively makes Rabbit Holes renewable. However, this only applies during Summer and only one Rabbit Hole will be regenerated at a time, while the others will remain queued. In other words, if 4 Rabbit Holes were to be dug up simultaneously, it would take 20 days of Summer to regenerate them all.
Putting a Trap directly above a Rabbit Hole will ensure a captured Rabbit most of the time.
In the base game, multiple traps can be placed after the first trap has caught a Rabbit, to stockpile Rabbits. This is useful during Winter or when one wishes to farm Nightmare Fuel when the Rabbits have turned into Beardlings.