Spider Queen

"She is regal in her horribleness."

- Wendy

Spider Queen is an aggressive Monster that spawns from overgrown tier 3 Spider Dens, if the player or other mobs wander close by. She has 1250 health and deals 80 damage per bite. This is the second "boss" type of monster added to the game (after the Treeguard and before Deerclops). She will also give birth to surface Spiders every 20 seconds, if the player is nearby; if the Queen has targeted the player, a third of these spiders will be Spider Warriors. A Queen can have a maximum of 16 followers. Those spiders will be automatically aggressive towards the player but will retreat back to the Queen if the player gets far away.

As a Queen emerges, she leaves a tier 1 Den in place (if there are fewer than 4 other Dens/Queens in vicinity), which quickly grows into a tier 2 Den (in 60 seconds), and can eventually spawn another Queen, thus making the Spiderhat a renewable item. Also, she can plant herself as a Tier 1 Den after wandering for 1.5 game days if she finds an open space away from other Dens/Queens.

With a normal damage dealing character it takes 39 hits with Spear, 30 with a Bat Bat, 25 with a Tentacle Spike, Ham Bat and Thulecite Club, and 19 with a Dark Sword to be killed. She takes four blasts from the Ice Staff to be frozen.

Kiting
The Queen is slow, so it is possible to attack her in the pauses between attacks. Always kill the hatched Spiders/Spider Warriors, though, before attempting to attack the Queen again. As always, good armor and weapons help a lot. If fighting without armor, having a couple of traps set around to kill the spawned spiders is a good idea. However, the Spider Queen can drain your sanity completely within thirty seconds, so it is advisable to keep your distance somewhat.

Pigs
Lure the Queen to a Pig Village, and let the Pigs attack her. Due to the run & hit combat behavior of the Pigs, she and her babies have trouble to hit them. As long as the Spiders she hatches are kept away from the Pigs, they should kill her quite quickly. This method also can prevent serious sanity drain.

Swamp Tentacles
If there happens to be a swamp nearby, lead the Queen to the swamp and over the Tentacles as they attack pretty much everything that passes by. Expect about 3-5 destroyed Tentacles in the process.

Beefalo
If you can find a herd of Beefalo in heat, and get the Queen close enough to them for at least one Beefalo to target it, the whole herd will eventually join in on the Queen. The Beefalo will not eat the meat dropped like pigs do. However, due to the fact that Beefalos don't use a kite method of attack like pigs, the Queen will stand her ground and be able to kill at least 3-4 Beefalos single-handedly.

Spiders
If the player has a Spiderhat, it is possible to gather spider allies from Spider Dens and order them to attack the Queen.

Bees
Lure it to a Killer Bee Hive as the Bees and Killer Bees have the same kiting technique as Pigs do. When a Killer Bee attacks the Queen, and it strikes back, all nearby bees will become aggressive to the Queen. You can also capture and trap bees using a Bug Net and release them near the Queen. Bee Mines are another option to ensure that the bees will attack the Queen.

Werebeaver
At the full moon, position Woodie (if you are playing as that character) near the Spider nest. He will transform into the Werebeaver, and attack the nest. This is preferable than to attacking as Woodie because of the Werebeaver's strength and health.

Blow Darts
This is the easiest way to defeat the Spider Queen. Make 13 Blow Darts and shoot the Spider Queen as fast as possible, without letting her or her Spiders catch up to you.

Gunpowder
The Spider Queen can be easily killed by freezing her with the Ice Staff and placing 5 gunpowders near her lighting them up and finishing her off by spear. If you kill her by placing 6 gunpowders, you will get from her just ash and Cooked Monster Meat, because of the fire damage as a result of the explosion

Bugs

 * Fighting other mobs in the presence of a Spider Queen will cause "E.F.S." (the boss monster song) to play. As long as the player is not within her (and her spawn's) "aggro radius", the Spider Queen does not become aggressive to the player. This also applies to fighting near a Treeguard.
 * Sometimes, when the Spider Queen is killed as she plants herself, she'll drop loot but still will leave a den.